Tickborne BBB Plus 3 day

The next generation of Lyme + Co-Infection Diagnostics—designed to detect at every stage

Combining direct and indirect testing methods, specifically for Bartonella infection, enhances diagnostic accuracy, especially for patients with complex clinical presentations. MosaicDX recommends maximizing diagnostic data by testing for both antibodies (indirect) and DNA evidence of infection (direct) for Bartonella, as each test method provides critical support. Tickborne BBB Direct Detect dPCR includes tests for Borrelia, Babesia and Bartonella at a genus level for all associated pathogens and Bartonella IgG Detect uses the IFA method to detect IgG to the top four (4) Bartonella species affecting humans in North America.

Tickborne BBB Direct Detect – dPCR (3 day) and Bartonella IgG Detect – IFA

Tickborne BBB Direct Detect dPCR, confirms current infection by directly detecting genus-level DNA for a broad range of Bartonella, Borrelia, and Babesia species (spp) in whole blood. This approach ensures that positive cases of infection with less common species are not missed.

Bartonella IgG Detect IFA confirms antibodies against the four most common species of infection: Bartonella henselae, Bartonella koehlerae, Bartonella quintana, and Bartonella vinsonii berkhoffii.

We offer nine (9) individual tests and combinations of the most common Lyme and co-infections in North America, with options designed to meet every practitioner’s need.

Blood, Serum
For Non-Practitioners:

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Patients presenting with flu-like symptoms, with or without Erythema migrans (EM) rash, and have lived or traveled to an area where Lyme disease is endemic.

Patients with chronic illness not improving, and past/present exposure to ticks, even with past treatment of Lyme, or Post-Treatment Lyme Disease Syndrome (PTLDS) or co-infections.

Patients with chronic illness not improving, and past/present exposure to ticks, fleas, lice, spiders or companion animals.

  • Abdominal pain
  • Anorexia
  • Anxiety and depression
  • Arthritis
  • Bladder and pelvic pain
  • Body aches
  • Cardiovascular issues (endocarditis)
  • Carditis or heart block
  • Changes in bowel habits
  • Depression
  • Eye and vision problems (such as floaters, light sensitivity, and blurry vision)
  • Facial pain and tooth issues
  • Fatigue / chronic fatigue / profound fatigue
  • Fever
  • Fibromyalgia
  • Flu-like illness
  • Headaches
  • Joint pain
  • Localized or disseminated EM
  • Malaise
  • Muscle pain / soreness
  • Muscle/limb impairment or paralysis
  • Neurological manifestations
  • Neurological symptoms such as facial paralysis, blindness
  • Psychiatric symptoms (rage)
  • Rash “striae”
  • Seizures
  • Sleeplessness
  • Swollen lymph nodes in the head, neck, arms, and along the shins

Details

MosaicDX offers nine (9) individual tests and combinations of the most common Lyme and co-infections in North America, with options designed to meet every practitioner’s need.

Combinations and Complementary Test Recommendations

Ticks, fleas and other vectors can transmit multiple infections—not just Bartonella. To help prioritize treatment, providers should consider additional Lyme and Co-infection tests to identify which pathogens are currently present.

Lyme Co-Infections Logo
Lyme Borrelia Direct Detect
Bartonella IgG Detect – IFA
Tickborne BBB Direct Detect
(1 or 3 day)

Methodology
Nanotrap®
Direct
Immuno-fluorescence (IFA)
Indirect
Digital PCR (dPCR)
Direct

Sample Type
Urine
Urine
Serum
Serum (yellow top)
Blood
Blood (lavender top)

INDIVIDUAL PROFILES

COMBINATION PROFILES

Analytes

Tickborne BBB Direct Detect – dPCR
Babesia spp. 3 days
Bartonella spp. 3 days
Borrelia spp. 3 days
Bartonella spp. Culture dPCR 3 days

Bartonella IgG Detect – IFA
Bartonella henselae
Bartonella koehlerae
Bartonella quintana
Bartonella vinsonii berkhoffii

Frequently Asked Questions

Have a question? We've got answers.

Our team of experts can help you find exactly what you need. Contact us now and let's get started.

Clinical References

  1. Maggi, R.G., Calchi, A.C., Moore, C.O. et al. Human Babesia odocoilei and Bartonella spp. co-infections in the Americas. Parasites Vectors 17, 302 (2024). https://doi.org/10.1186/s13071-024-06385-4. 
  1. Hildebrand, J., Buńkowska-Gawlik, K. & Perec-Matysiak, A. Co-occurrence of Babesia microti, Bartonella spp., Borrelia burgdorferi s.l. and Anaplasma phagocytophilum in rodents from Lower Silesia, Poland. Parasites Vectors 7 (Suppl 1), O4 (2014). https://doi.org/10.1186/1756-3305-7-S1-O4
  1. Breitschwardt E. The Evolving Biomedical Importance of Bartonella Species Infections. Pathogens. Special Issue. Bacterial Pathogens. ISSN 2076-0817. Accessed May 2025. 
  1. Infectious Disease Society of America 2020 Guideline on Diagnosis and Management of Babesiosis. Clinical Infectious Diseases, Volume 72, Issue 2, 15 January 2021, Pages e49-e64, https://doi.org/10.1093/cid/ciaa1216. 

  1. Spach, David. UpToDate. Biology of Bartonella Species. Accessed 05/2025. 
  1. Breitschwerdt E. Bartonellosis: One Health Perspectives for an Emerging Infectious Disease. ILAR Journal, Volume 55, Issue 1, 2014, Pages 46–58, https://doi.org/10.1093/ilar/ilu015 
  1. Bush J, Robveille C, Maggi R, et al. Bush, J.C., Robveille, C., Maggi, R.G. et al.Neurobartonelloses: emerging from obscurity!Parasites Vectors17, 416 (2024). https://doi.org/10.1186/s13071-024-06491-3 
  1. Maggi, R.G., et al. (2005). Bartonella spp. bacteremia in patients with neurological and neurocognitive dysfunction. Journal of Clinical Microbiology, 43(9), 4818-4822. 
  1. Breitschwerdt, E.B., et al. (2008). Bartonella spp. bacteremia and rheumatic symptoms in patients from Lyme disease-endemic region. Emerging Infectious Diseases, 14(5), 653-660. 
  1. Breitschwerdt E, Atkins C, Brown T, et al. Bartonella vinsonii subsp. Berkhoffi and Related Members of the Alpha Subdivision of the Proteobacteria in Dogs with Cardiac Arrhythmias, Endocarditis, or Myocarditis. Journal of Clinical Microbiology. Nov 1999, Vol 37 No 11: 3618-26. 
  1. Cheslock M, and Embers M. Human Bartonellosis: An Underappreciated Public Health Problem? Tropical Medicine and Infectious Disease Review April 2019. 
  1. Boaz A, Graidy M, Efrat G, et al. Bartonella Koehlerae, a New Cat-Associated Agent of Culture-Negative Human Endocarditis. Journal of Clinical Microbiology, Aug. 2004, v42 No 8: 3462–3468. 
  1. Project Lyme. What is Bartonella. Accessed May 2025. 
  1. Centers for Disease Control and Prevention. About Bartonella Henselae. Accessed May 2025. 
  1. Galaxy Diagnostics. What is Bartonellosis. Accessed May 2025. 

  1. Shapiro, Eugene. Borrelia burgdorferi (Lyme Disease). Pediatr Rev (2014) 35 (12): 500–509. https://doi.org/10.1542/pir.35-12-500 
  1. Pfeifle A, Duvall, R, Tamming L, et al. Borrelia burgdorferi Strain-Specific Differences in Mouse Infectivity and Pathology. Pathogens 2025, 14(4), 352; https://doi.org/10.3390/pathogens14040352
  1. Centers for Disease Control and Prevention. Comparison of Lyme Disease in the United States and Europe. Emerging Infectious Diseases. Vol. 27, Number 8—August 2021 
  1. Wilcox J, Mankoff S, Stricker J. Severity of chronic Lyme disease compared to other chronic conditions: a quality of life survey. PeerJ. 2014; 2:e322. PMID: 24749006 PMCID: PMC3976119. 
  1. Sellati, T, and Barberio D. Mechanisms of Dysregulated Antibody Response in Lyme Disease. Front Cell Infect Microbiol. 2020 Oct 7;10:567252. doi: 10.3389/fcimb.2020.567252. 
  1. Magni R, Espina B, Shah K, et al. Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosis. J Transl Med. 2015 Nov 4;13:346. doi: 10.1186/s12967-015-0701-z. 
    Aucott, J, Rebman A, et al. Post-treatment Lyme disease syndrome symptomatology and the impact on life functioning: is there something here? Qual Life Res. 2012 Feb 1;22(1):75–84. doi: 10.1007/s11136-012-0126-6
  2. International Lyme and Associated Diseases Society (ILADS). Lyme Disease Basics For Providers. https://www.ilads.org/research-literature/lyme-disease-basics-for-providers/. Accessed May 2025.
  3. Centers for Disease Control and Prevention (CDC). How Lyme Disease Spreads. https://www.cdc.gov/lyme/causes/index.html. Accessed May 2025.
  4. Melski JW. Lyme borreliosis. Semin Cutan Med Surg. 2000 Mar;19(1):10-8. doi: 10.1053/sd.2000.7373. PMID: 10834603.
  5. Kurokawa, C., Lynn, G.E., Pedra, J.H.F. et al. Interactions between Borrelia burgdorferi and ticksNat Rev Microbiol 18, 587–600 (2020). https://doi.org/10.1038/s41579-020-0400-5.
  6. Infectious Disease Society of America (IDSA). AAN/ACR/IDSA 2020 Guidelines for the Prevention, Diagnosis and Treatment of Lyme Disease. Clinical Infectious Diseases, Volume 72, Issue 1, 1 January 2021, Pages e1-e48, https://doi.org/10.1093/cid/ciaa1215
  7. Cameron D, Johnson L, Maloney E. International Lyme and Associated Diseases Society (ILADS). Evidence Assessments and Guideline Recommendations in Lyme Disease: The Clinical Management of Known Tick Bites, Erythema Migrans Rashes and Persistent Disease. https://www.ilads.org/patient-care/ilads-treatment-guidelines/. Accessed May 2025.