Collection Instructions

Urine Sample Collection Instructions

Publish Date

August 4, 2026

Overview

Visual, step-by-step test prep and collection instructions for urine specimen collection.

 

Collection Instructions: Urine

Test Kit Contents

Start by identifying these elements of your test kit. If you are missing or have expired components, contact Mosaic Customer Service at 800-288-0383.

  • Test kit box
  • Silver thermo bag
  • Ice pack (place flat in freezer immediately)
  • Clear biohazard zip-lock bag
  • Absorbent packing sheet
  • Vial (Only required if completing the Amino Acids Test)
  • Collection cup (empty cup)
  • Shipping laboratory pak

Precaution

Amino acids only. Keep the kit contents out of the reach of children and pets. Do not spill preservative fluid in the vials and avoid contact with skin or eyes. For eye contact flush with water thoroughly for 15 minutes. For skin contact wash thoroughly with soap and water. For accidental ingestion contact poison control immediately. Safety Data Sheets

Before You Start

  • Plan to collect your first morning urine sample.
  • Limit fluid intake after 6:00 pm the night prior to collection.
  • Prior to bed, empty your bladder. Upon waking, collect your urine prior to eating or drinking. Ensure that it has been at least 6 hours since you emptied your bladder.
  • A diluted urine sample may be inadequate for analysis and may be rejected.
  • Samples visibly contaminated with blood (red, pink, or brown in color) will be rejected.
  • Consult your healthcare provider before stopping any medications. Discontinuing supplements three days prior to specimen collection is recommended unless otherwise directed by your provider.
  • Samples must be shipped Monday through Thursday (outside the US: Monday or Tuesday). If you collect on the weekend, hold the specimen until Monday to ship.

Preparing For Your Test

Important: Do not discontinue medications or supplements without first consulting your healthcare practitioner.

Test Requirements

Test Requirements / Notes
Organic Acids Test (OAT) and Microbial Organic Acids Test (MOAT) Minimum 10 mL. For 48 hours before collection avoid apples, grapes, raisins, pears, cranberries, and their juices or products; also avoid arabinogalactan, echinacea, reishi mushrooms, and ribose supplements. Wait 48 hours after completing a course of antibiotics.
MycoTOX Profile Minimum 10 mL. Fasting for 12 hours may increase excretion of mycotoxins from adipose tissue (not required). At least 3 days prior, avoid glutathione, charcoal, bentonite clay, or similar binders. Samples cannot be tested for patients currently taking Mycophenolate (CellCept/Myfortic) — consult with your practitioner on when, or if, you can stop taking this medication for at least 72 hours before collection.
TOXDetect Minimum 10 mL. If taking Cephalexin (Cefalexin, Keflex, Bio-Cef, Novo-Lexin), wait a minimum of four days after the last dose.
Glyphosate Minimum 10 mL. No special prep.
Calcium + Magnesium Minimum 10 mL. No special prep.
Metals Urine (random) Minimum 10 mL. It is recommended to omit fish and shellfish for one week prior to collecting. If an MRI containing Gadolinium-based media has been administered, a urine specimen should not be collected for a minimum of 96 hours.
Amino Acids Urine Test Minimum 10 mL. Discontinue any amino acid supplements 48 hours prior to collection. If you are taking antibiotic medications, please finish the course of medication, and then wait 48 hours before collection. Pour urine into white-top vial to the fill line; leave 5 mL in the cup. Tighten both lids and invert vial 5x to mix. Freeze both until shipment.

After Collection

  • Screw lid on specimen container tightly.
  • Freeze urine specimen for a minimum of 4 hours before shipping. Keep in freezer until ready to ship.

Let’s Get Started

  1. Complete the information on the barcode sticker AND the specimen container(s) by writing the patient name, date of birth, collection date and time, and your initials. If this information is missing, your sample will be rejected..
  2. Place the barcode sticker on the specimen container(s). Be careful not to put the sticker over the information you already wrote on the cup. Use blue or black ink from a ball point pen. Do not use felt-tipped pens or dry erase markers. Remember: Your sample can’t be tested without patient information on the cup.
  3. Remember: Your sample can’t be tested without patient information on the cup.
  4. Collect a first morning urine specimen and tighten the lid on collection cup until you hear two clicks. This indicates the container is securely closed and ready for safe transport. Urine must be yellow in color. If urine is clear the sample is too diluted and may be rejected.

After Collection

  1. Freeze Urine specimen for a minimum of 4 hours before shipping. Keep specimen in the freezer until ready to ship.
  2. Preparing the Package

    1. Ensure frozen specimen(s) are labeled and tightly sealed. Check the box indicating it has been frozen.
    2. Place specimen(s) and absorbent packing sheet into the biohazard zip-lock bag and seal.
    3. Place sealed biohazard bag and frozen ice pack into the silver thermo bag and seal.
    4. Place the filled silver thermo bag into the test kit box and close the lid.
    5. Locate the shipping instruction card for details on how to ship.
    6. Samples must be shipped Monday through Thursday (outside the US: Monday or Tuesday). If you collect on the weekend, hold the specimen until Monday to ship.

    Any Questions?

    If you have questions about the specimen collection or shipping process, please contact us:

    Phone: 800-288-0383 — Customer service is available Monday–Friday, 8am–5pm CST.
    Email: CustomerService@MosaicDX.com

    Turnaround: Your test results will be delivered to your healthcare practitioner within 2–3 weeks after Mosaic Diagnostics receives the sample(s). Your practitioner will review results and discuss next steps. Mosaic Diagnostics personnel cannot discuss test results directly with patients or family members.